Evidence the pp60src, the product of the Rous sarcoma virus src gene, undergoes autophosphorylation

Abstract

Pp60src, the product of the Rous sarcoma virus src gene, was purified > 100,000-fold by a combination of ion-exchange and immunoaffinity chromatography. Incubation of pp60src purified in this fashion with [32P-.gamma.]ATP resulted in a single 32P-labeled protein when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Staining of these gels with AgNO3 showed a predominant 60,000-dalton polypeptide which comigrated with the protein labeled with 32P in vitro. Partial digestion of this protein with staphylococcal V8 protease after in vitro iodination indicated that it was pp60src. Apparently, pp60src is able to autophosphorylate.