Androgen receptors in cultured human skin fibroblasts
- 1 November 1982
- journal article
- Published by Oxford University Press (OUP) in British Journal of Dermatology
- Vol. 107 (s23), 40-46
- https://doi.org/10.1111/j.1365-2133.1982.tb01030.x
Abstract
Human skin may be considered as a target organ for androgens, since events characteristic of androgen action have been described in this tissue, as well as in cultured human skin fibroblasts. The culture of human skin fibroblasts gives the opportunity to work on living cells, under controlled conditions, in a renewable material from a single skin biopsy. Using this method, we showed the presence od DHT-receptors in the human fetus and we studied the ontogenesis of androgen receptors in relation to sexual differentiation. In the neonatal period, the physiological T rise was not concurrent with a variation in sex skin androgen receptors. The evolution of DHT binding during puberty is now under investigation. These data suggest that the androgen receptor is not modulated by plasma androgens. Androgen receptor determination is of value in defining the biochemical defects involved in partial and complete androgen insensitivity syndrome (twenty-six cases in our study). In idiopathic hirsutism, DHT binding is used to detect an eventual local hypersensitivity. Fibroblast cultures have also been shown to be an excellent model for the screening of compounds which might block the expression of androgen action by competing for the androgen receptors. Cultured skin fibroblasts are a valuable model for the study of androgen and antiandrogen action in human skin.Keywords
This publication has 10 references indexed in Scilit:
- Inhibition of androgen binding in human foreskin fibroblasts by antiandrogensSteroids, 1981
- The Ontogeny of the Androgen Receptor in Human Foreskin*Journal of Clinical Endocrinology & Metabolism, 1981
- Cimetidine competition with androgens for binding to human sex skin fibroblasts androgen receptorsThe Journal of Steroid Biochemistry and Molecular Biology, 1980
- METABOLISM AND ACTION OF ANDROGEN IN THE SKINInternational Journal of Dermatology, 1979
- Role of testosterone binding to the androgen receptor in male sexual differentiation of patients with 5α-reductase deficiencyThe Journal of Steroid Biochemistry and Molecular Biology, 1979
- Selective inhibition by secosteroids of 5α-reductase activity in human sex skin fibroblastsSteroids, 1979
- Pathogenesis of the complete androgen insensitivity syndrome (AIS) with normal dihydrotestosterone (DHT) receptor bindingPediatric Research, 1978
- Androgen receptor in human skinBritish Journal of Dermatology, 1977
- Androgen receptor in human skin fibroblasts characterization of a specific 17β-hydroxy-5α-androstan-3-one-protein complex in cell sonicates and nucleiSteroids, 1975
- Evidence for an Androgen-independent Mechanism Regulating the Levels of Receptor in Target TissueJournal of Biological Chemistry, 1973