Strain differences in rat liver (UDP-glucuronyltransferase activity towards androsterone
- 1 June 1979
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 179 (3), 483-487
- https://doi.org/10.1042/bj1790483
Abstract
Male Donryu, Wistar King rats showed discontinuous variations in hepatic microsomal UDP-glucuronyltransferase activities towards androsterone, but not towards testosterone, bilirubin, phenolphthalein and 4-nitrophenol. Fresh microsomal fraction with a low transferase activity towards androsterone formed 0.049–0.080 nmole of glucuronide/min per mg of protein, whereas fresh microsomal fraction with a high transferase activity towards androsterone formed 0.335–0.557 nmol of glucuronide/min per mg of protein. The microsomal fraction with low enzyme activity towards androsterone was not stimulated by treatment with Triton X-100 or freezing and thawing. In contrast, male Long Evans and Sprague-Dawley rats did not exhibit such diversity.This publication has 14 references indexed in Scilit:
- Discontinuous variation in hepatic uridine diphosphate glucuronyltransferase toward androsterone in Wistar ratsBiochemical Pharmacology, 1979
- Substrate specificity and properties of uridine diphosphate glucuronyltransferase purified to apparent homogeneity from phenobarbital-treated rat liverBiochemical Journal, 1978
- Functional heterogeneity of UDP-glucuronosyltransferase as indicated by its differential development and inducibility by glucocorticoids. Demonstration of two groups within the enzyme's activity towards twelve substratesBiochemical Journal, 1978
- Reactivation of a pure defective UDP‐glucuronyltransferase from homozygous gunn rat liverFEBS Letters, 1978
- Steroid and non-steroid UDP glucuronyltransferase: Glucuronidation of synthetic estrogens as steroidsThe Journal of Steroid Biochemistry and Molecular Biology, 1977
- Separation and partial purification of two differentially inducible UDP-glucuronyltransferases from rat liverBiochemical Pharmacology, 1977
- Studies on the properties of an enzyme forming the glucuronide of pregnanediol and the pattern of development of steroid glucuronyltransferases in rat liverJournal of Steroid Biochemistry, 1977
- Biliary metabolites of testosterone and testosterone glucosiduronate in the ratSteroids, 1974
- Determination of bilirubin glucuronide and assay of glucuronyltransferase with bilirubin as acceptorBiochemical Journal, 1968
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951