« Spontaneous » Endoreduplication inChinese HamsterCell Cultures. II. Analysis of the Mitotic Cell Cycle

Abstract

Endoreduplicated cells appear in sub-cultures prepared by trypsinising cultures of Chinese Hamster in the plateau phase, which after a change of medium are grown for at least two days, in stoppered bottles. E-cells perform the first DNA synthesis before trypsinisation and the second after it; therefore these cells were blocked or slowed down at the moment of trypsinisation in a stage of the cycle with 4C of DNA. This stage is probably comparable to a G. stage that follows a first cell cycle that has concluded without a normal mitosis.