Resolution, reconstitution and kinetics of the primary action of a hormone receptor

Abstract
The .beta.-adrenergic receptor (R) and GTP binding protein (G) with which the receptor interacts were obtained in separate soluble fractions. Their recombination produced a hormone [isoprenaline] responsive system. In the presence of hormone the encounter of R with G in the reconstituted membrane of turkey erythrocytes occurs much faster than the subsequent R-induced activation of G.