A Rapid Bulk Nissl Method

Abstract

A simplified and time-saving Nissl method is descr. in which the fresh nervous tissue is placed directly into: dioxane, 65-70 ml.; water, 15 ml.; 95% alc., 20-15 ml.; and toluidine blue, 1 g. This soln. fixes, stains, partially clears, and dehydrates in one procedure. The material is then sectioned according to a dry-ice method and mounted on the slide either with or without the use of the rapid albumen procedure. Thus it is possible to terminate an expt. one day and have the tissue ready for microscopic observation on the next.