Unterscheidung von Kallikreinen und Kallikreinvorstufe mittels Antiseren und Diisopropylfluorophosphats

Abstract

Pig pancreatic kallikrein initiates antibody formation when injected into rabbits. Diisopropylfluorophosphate (DFP) blockage of the active centre does not alter its antigenic properties. Pancreatic kallikrein and serum kallikrein from pig are, however, immunologic ally different. The increased inhibition of human serum kallikrein by anti-human serum from rabbits could be due to an immunological similarity between serum kallikreinogen and serum kallikrein. Serum kallikreinogen, however, differs from serum kallikrein by its resistance to DFP. Thus inhibition of kallikrein by DFP depends on the availability of the active center, while the immunological specificity is determined by other parts of the molecule.