Characteristics of Maize Stripe Virus Transmission by the Corn Delphacid12

Abstract

The relationship between maize stripe virus (MStpV) and its insect vector Peregrinus maidis (Ashmead) is described. The virus is transmitted by P. maidis in a persistent manner. 2nd to 3rd instars transmitted MStpV with ca. twice the efficiency after a 24-, 48-, 6R-, 96-, and 192-h acquisition access period (AAP) as did adults. Macropterous adults were slightly more efficient transmitters than brachypterous adults. The minimum AAP of the virus by 2nd and 3rd instars is 4 h. Longer AAP increased the rate of transmission. The minimum incubation period (IP) in both nymphs and adults is 4 to 5 days. The median IP in both age groups was independent of AAP. P. maidis nymphs retained inoculativity much longer than adults. The average retention of MStpV by 2nd to 3rd instars was 13.7, 13.2, and 15.8 days after 48.72, and 96 h AAP. whereas the average retention period in adults was 3.7, 5.5, and 6.5 days, respectively. It was demonstrated that MStpV could be transmitted by hemolymph injection and transovarial passages.