Studies on the Mechanism of the Enzymatic Action of Taka-amylase-A

Abstract

Taka-amylase A could not hydrolyze phenyl monoacetyl-alpha-maltoside (O-alpha-D-glucopyranosyl-(l[forward arrow]4)-phenyl-6-O-acetyl-alpha-D-glucopyranoside). The monoacetyl derivative did not inhibit hydrolysis of phenyl alpha-maltoside by taka-amylase A. Hydroxyl group at the C-6 position is essential for forming the enzyme-substrate complex.