Subtilases: The superfamily of subtilisin-like serine proteases
- 1 March 1997
- journal article
- review article
- Published by Wiley in Protein Science
- Vol. 6 (3), 501-523
- https://doi.org/10.1002/pro.5560060301
Abstract
Subtilases are members of the clan (or superfamily) of subtilisin‐like serine proteases. Over 200 subtilases are presently known, more than 170 of which with their complete amino acid sequence. In this update of our previous overview (Siezen RJ, de Vos WM, Leunissen JAM, Dijkstra BW, 1991, Protein Eng 4:719–731), details of more than 100 new subtilases discovered in the past five years are summarized, and amino acid sequences of their catalytic domains are compared in a multiple sequence alignment. Based on sequence homology, a subdivision into six families is proposed. Highly conserved residues of the catalytic domain are identified, as are large or unusual deletions and insertions. Predictions have been updated for Ca2+‐binding sites, disulfide bonds, and substrate specificity, based on both sequence alignment and three‐dimensional homology modeling.Keywords
This publication has 84 references indexed in Scilit:
- Structural analysis and proteolytic activation of Enterococcus faecalis cytolysin, a novel lantibioticMolecular Microbiology, 1996
- Structure‐function studies on the biosynthesis and bioactivity of the precursor convertase PC2 and the formation of the PC2/7B2 complexFEBS Letters, 1995
- Alignment/Phylogeny of the Papain Superfamily of Cysteine ProteasesJournal of Molecular Biology, 1995
- Increasing thermal stability of subtilisin from mutations suggested by strongly interacting side-chain clustersProtein Engineering, Design and Selection, 1995
- Purification and characterization of a serine proteinase from senescent sporophores of the commercial mushroom Agaricus bisporusJournal of General Microbiology, 1993
- Refined crystal structures of subtilisin Novo in complex with wild-type and two mutant eglinsJournal of Molecular Biology, 1991
- Furin is a subtilisin-like proprotein processing enzyme in higher eukaryotesMolecular Biology Reports, 1990
- Basic local alignment search toolJournal of Molecular Biology, 1990
- Dictionary of protein secondary structure: Pattern recognition of hydrogen‐bonded and geometrical featuresBiopolymers, 1983
- On the size of the active site in proteases. I. PapainBiochemical and Biophysical Research Communications, 1967