Mapping of restriction sites in the attachment site region of bacteriophage lambda
- 1 January 1977
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 154 (3), 231-248
- https://doi.org/10.1007/bf00571278
Abstract
A fine structure map of theEcoRI fragment containing the lambda attachment-site region has been constructed. 38 different restriction endonucleases have been employed and 170 sites located in this fragment. In addition, sites in adjacent regions have been determined for several enzymes. Complete cleavage maps of the entire lambda genome have been obtained for endonucleasesBglII,BluI,KpnI,SacI,SacII,SalI andXbaI. The strategy employed for mapping included comparison of deletion and substitution mutants, analysis of mixed digests, and detailed analysis of subfragments.Keywords
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