Determination of 8-Methoxypsoralen, A Photoactive Compound, in Blood by High Pressure Liquid Chromatography
- 1 January 1977
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 10 (1), 39-50
- https://doi.org/10.1080/00032717708059185
Abstract
A rapid, sensitive, and specific high pressure liquid chromatographic (HPLC) assay was developed for the determination of 8-methoxypsoralen (8-MOP) from blood. The assay involves extraction of 8-methoxypsoralen into n-hexane: 2-propanol (95:5) from blood buffered to pH 9. The overall recovery is 75 ± 5.0% (S.D.), and the sensitivity limit of detection is 10–15 ng/ml of blood, using a 1 ml specimen. The assay was used in the determination of blood levels of 8-methoxypsoralen in a dog following intravenous and oral administration of a 5 mg/kg dose, and in man following single oral 0.56 mg/kg doses of 8-MOP, totalling 40 mg.Keywords
This publication has 4 references indexed in Scilit:
- Photochemotherapy for psoriasis with orally administered methoxsalenArchives of Dermatology, 1976
- Simplification of local therapy of psoriasis with 8-methoxypsoralen.British Journal of Dermatology, 1976
- Photochemotherapy of Psoriasis with Oral Methoxsalen and Longwave Ultraviolet LightNew England Journal of Medicine, 1974
- Determination medazepam (Nobrium), diazepam (Valium), and their major biotransformation products in blood and urine by electron capture gas-liquid chromatographyAnalytical Chemistry, 1970