The binding of nicotinamide-adenine dimucleotide to glyceraldehyde 3-phosphate dehydrogenase from Bacillus stearothermophilus
- 1 December 1975
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 151 (3), 747-749
- https://doi.org/10.1042/bj1510747
Abstract
The binding of NAD+ to glyceraldehyde 3-phosphate dehydrogenase (EC 1.2.1.12) from Bacillus stearothermophilus has been studied by measurement of protein fluorescence quenching. Slight negative co-operativity was observed in the binding of the third and fourth coenzyme molecules to the tetrameric enzyme. The first two coenzyme molecules were tightly bound. In this respect the enzyme resembles that from sturgeon muscle rather than that from yeast.Keywords
This publication has 14 references indexed in Scilit:
- Binding of NAD+ and NADH to rabbit-muscle glyceraldehydephosphate dehydrogenaseBiochimica et Biophysica Acta (BBA) - Enzymology, 1971
- Co-operative binding of nicotinamide-adenine dinucleotide to yeast glyceraldehyde-3-phosphate dehydrogenase: I. Equilibrium and temperature-jump studies at pH 8.5 and 40 °CJournal of Molecular Biology, 1971
- The binding of NAD+ to rabbit muscle glyceraldehyde-3-phosphate dehydrogenase studied by protein fluorescence quenchingBiochimica et Biophysica Acta (BBA) - Enzymology, 1971
- Thermodynamics of nicotinamide-adenine dinucleotide addition to the glyceraldehyde 3-phosphate dehydrogenases of yeast and of rabbit skeletal muscle. An equilibrium and calorimetric analysis over a range of temperaturesBiochemistry, 1971
- Positive and negative cooperativity in yeast glyceraldehyde 3-phosphate dehydrogenaseBiochemistry, 1970
- Binding and properties of NAD+ in glyceraldehydephosphate dehydrogenase from lobster-tail muscleBiochimica et Biophysica Acta (BBA) - Enzymology, 1969
- The Amino Acid Composition and Other Properties of Thermostable Glyceraldehyde 3-Phosphate Dehydrogenase from Bacillus stearothermophilusJournal of Biological Chemistry, 1969
- Negative cooperativity in enzyme action. Binding of diphosphopyridine nucleotide to glyceraldehyde-3-phosphate dehydrogenaseBiochemistry, 1968
- The reaction between NAD+ and rabbit-muscle glyceraldehydephosphate dehydrogenaseBiochimica et Biophysica Acta (BBA) - Enzymology, 1968
- The Purification of Nicotinamide Adenine Dinucleotide and the Kinetic Effects of Nucleotide ImpuritiesJournal of Biological Chemistry, 1963