Detection of non-genotoxic carcinogens in the BALB/c 3T3 cell transformation/mutation assay system
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Mutagenesis
- Vol. 4 (4), 286-291
- https://doi.org/10.1093/mutage/4.4.286
Abstract
We have employed BALB/c 3T3 cells in a simultaneous cell transformation and mutation assay protocol to see whether both genotoxic and non-genotoxic carcinogens can be identified. Three known mutagenic animal carcinogens tested positive for transforming activity: dimethylnitrosamine, methylcholanthrene and methylnitrosourea. In addition, we tested three non-mutagenic compounds-two human carcinogens, benzene and diethylstilbestrol, and the possible human carcinogen dichlorodiphenyltrichloroethane; all three displayed transforming activity. These results add to existing data supporting the validity of the BALB/c 3T3 cell transformation system as a reliable short-term in vitro test for carcinogens in general, and for non-genotoxic carcinogens in particular.This publication has 16 references indexed in Scilit:
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