Antibody-Mediated enhancement of BK virus infection in human monocytes and a human macrophage-like cell line

Abstract

We have monitored BK virus (BKV) antigen expression and multiplication in human monocytes and in a human macrophage (Mo)-like cell line (U937) in the presence or absence of dilution series of human or rabbit anti-BKV antisera. After infection with BKV alone, restricted expression (structural antigens and T-antigen) and multiplication was recorded in monocytes from some donors, while in U937 cells and monocytes from other donors, no signs of viral activity were detected. Monocyte cultures established from the same donor at different times demonstrated antigen expression/multiplication on two occasions but not on the third. A pronounced enhancement of BKV expression/multiplication in human monocytes and multiplication in U937 cells was seen with some dilutions of all antisera (human and rabbit) used. The pattern of enhancement and the dilution resulting in maximum viral activity was constant and seemed to be determined by the serum, but the exact level of enhancement for a given serum differed considerably in monocytes from different donors and seemed to be determined by the cells. In the latter respect, monocytes taken from the same donor some weeks apart showed variations at the same level, as did cells from different donors. PMA (phorbol-12-myristate-13-acetate) stimulation of monocytes and U937 cells resulted in stronger antibody enhancement in terms of infectivity, without affecting the number of monocytes showing antigen expression. No expression/multiplication of BKV was detected in the murine Mo-like cell line P338 DI.