Endothelial growth factor present in tissue culture of CNS tumors
- 1 March 1976
- journal article
- research article
- Published by Journal of Neurosurgery Publishing Group (JNSPG) in Journal of Neurosurgery
- Vol. 44 (3), 342-346
- https://doi.org/10.3171/jns.1976.44.3.0342
Abstract
Human endothelial cells obtained from postpartum umbilical veins and placed in primary tissue cultures were treated with media from cultures of human and experimental CNS tumors. Endothelial proliferaton was determined by the uptake of 3H thymidine with autoradiography and represented as the thymidine labeling index (TI), which is the proportion of 3H thymidine-labeled endothelial cells to total number of cells counted. There was a marked increase in the TI when tumor-conditioned medium was added to endothelial cultures (range 28.7%-98.3%) when compared to controls (2.1%) and endothelium with conditioned media from fibroblasts (4.5%). This study demonstrates the presence of a chemical substance produced by tumor cells which results in endothelial proliferation. The system described provides a useful assay technique for the further characterization of this endothelial growth factor.This publication has 18 references indexed in Scilit:
- HUMAN VASCULAR ENDOTHELIAL CELLS IN CULTUREThe Journal of cell biology, 1974
- Culture of Human Endothelial Cells Derived from Umbilical Veins. IDENTIFICATION BY MORPHOLOGIC AND IMMUNOLOGIC CRITERIAJCI Insight, 1973
- Isolation of the nonhistone proteins of rat Walker carcinoma 256. Their association with tumor angiogenesisBiochemistry, 1973
- DNA synthesis in endothelium of aortic segments in vitroExperimental Cell Research, 1972
- TUMOR DORMANCY IN VIVO BY PREVENTION OF NEOVASCULARIZATIONThe Journal of Experimental Medicine, 1972
- TUMOR ANGIOGENESISThe Journal of cell biology, 1972
- Anti-AngiogenesisAnnals of Surgery, 1972
- Tumor Angiogenesis: Therapeutic ImplicationsNew England Journal of Medicine, 1971
- ISOLATION OF A TUMOR FACTOR RESPONSIBLE FOR ANGIOGENESISThe Journal of Experimental Medicine, 1971
- The structure of the blood vessels in cerebral tumoursThe Journal of Pathology and Bacteriology, 1940