Evidence for the occurrence of γ-methylene-α-oxoglutaric acid in groundnut plants (Arachis hypogaea)
- 1 February 1955
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 59 (2), 228-234
- https://doi.org/10.1042/bj0590228
Abstract
Keto acids present in extracts of groundnut seedlings (Arachis hypogaea) were investigated and characterized by paper chromatography of their 2:4-dinitrophenylhydrazine (DNPH) derivatives. alpha -Oxoglutaric, glyoxylic, pyruvic acids and a new keto acid were identified, and 12 mg of the DNPH derivative of the new acid were isolated from 2 kg of seedlings. Using a variety of physical and chemical methods, the DNPH derivative of the new keto acid was shown to be identical with the corresponding derivative of gamma-methylene-alpha-oxoglutaric acid (HOOC.C(:CH2). CH2.CO.COOH) prepared by biological transamination from the analogous amino acid.Keywords
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