Tissue‐specific expression of the human laminin α5‐chain, and mapping of the gene to human chromosome 20q13.2‐13.3 and to distal mouse chromosome 2 near the locus for the ragged (Ra) mutation

Abstract

To investigate the function of the laminin α5-chain, previously identified in mice, cDNA clones encoding the 953-amino-acid carboxy terminal G-domain of the human laminin α5-chain were characterized. Northern blot analysis showed that the laminin α5-chain is expressed in human placenta, heart, lung, skeletal muscle, kidney, and pancreas. The human laminin α5-chain gene (LAMA5) was assigned to chromosome 20q13.2-q13.3 by in situ hybridization, and the mouse gene (Lama5) was mapped by linkage analysis to a syntonic region of distal chromosome 2, close to the locus for the ragged (Ra) mutation.