Multiplex Genome Engineering Using CRISPR/Cas Systems
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Open Access
- 15 February 2013
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 339 (6121), 819-823
- https://doi.org/10.1126/science.1231143
Abstract
Genome Editing: Clustered regularly interspaced short palindromic repeats (CRISPR) function as part of an adaptive immune system in a range of prokaryotes: Invading phage and plasmid DNA is targeted for cleavage by complementary CRISPR RNAs (crRNAs) bound to a CRISPR-associated endonuclease (see the Perspective by van der Oost ). Cong et al. (p. 819 , published online 3 January) and Mali et al. (p. 823 , published online 3 January) adapted this defense system to function as a genome editing tool in eukaryotic cells.Keywords
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