Levels of Free Granulocyte Elastase in Bronchial Secretions from Patients with Cystic Fibrosis: Effect of Antimicrobial Treatment Against Pseudomonas aeruginosa
- 1 May 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Infectious Diseases
- Vol. 153 (5), 902-909
- https://doi.org/10.1093/infdis/153.5.902
Abstract
Large amounts of free granulocyte elastase (GE), an enzyme capable of mediating airway damage, have been found in bronchial secretions of patients with cystic fibrosis who are infected with Pseudomonas aeruginosa. This finding indicates an imbalance between GE and its antiproteases, α1-proteinase inhibitor (α1-PI) and bronchial mucosal inhibitor (BMI), in the airways of these individuals. The effect of intravenous antimicrobial treatment against P. aeruginosa on activity and concentration of GE, BMI, and α1-PI was evaluated in 30 treatment courses of 20 patients with cystic fibrosis. Although sputum volume and level of immunoreactive GE decreased and concentrations of α1-PI and BMI increased significantly (P < .05), a high level of free GE persisted. No active α1-PI and BMI were detectable after treatment. High levels of GE correlated with a poor pulmonary condition (rs = .98, P < .001). In vitro, elastolytic activity of bronchial secretions from patients with cystic fibrosis was significantly inhibited by eglin C and an oxidation-resistant variant of α1-PI, both compounds currently produced by recombinant DNA technology.Keywords
This publication has 23 references indexed in Scilit:
- A Radioimmunoassay for Measurement and Characterization of Human Antileukoprotease in SerumHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1981
- Potential Mediator of InflammationJCI Insight, 1980
- DEMONSTRATION OF A LOCAL EXHAUSTION OF COMPLEMENT COMPONENTS AND OF AN ENZYMATIC DEGRADATION OF IMMUNOGLOBULINS IN PLEURAL EMPYEMA - A POSSIBLE FACTOR FAVORING THE PERSISTENCE OF LOCAL BACTERIAL-INFECTIONS1980
- Pseudomonas Aeruginosa Allergy in Cystic FibrosisAllergy, 1980
- Mapping the extended substrate binding site of cathepsin G and human leukocyte elastase. Studies with peptide substrates related to the alpha 1-protease inhibitor reactive site.Journal of Biological Chemistry, 1979
- PROTEOLYSIS OF HUMAN-IGG BY HUMAN POLYMORPHONUCLEAR LEUKOCYTE ELASTASE PRODUCES AN FC-FRAGMENT WITH INVITRO BIOLOGICAL-ACTIVITY1979
- Studies on the Influence of Trasylol on the Partition of Trypsin between the Human Plasma Protease Inhibitors in vitroBiological Chemistry, 1978
- Immunoreactive Granulocyte Elastase in Human SerumHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1978
- PSEUDOMONAS-AERUGINOSA INFECTION IN CYSTIC-FIBROSIS - DIAGNOSTIC AND PROGNOSTIC SIGNIFICANCE OF PSEUDOMONAS-AERUGINOSA PRECIPITINS DETERMINED BY MEANS OF CROSSED IMMUNOELECTROPHORESIS1977
- Isolation and Characterisation of a Low Molecular Weight Inhibitor (of Chymotrypsin and Human Granulocytic Elastase and Cathepsin G) from LeechesHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1976