Chromosome Banding Patterns in Ehrlich and Yoshida Ascites Tumors2

Abstract

Chromosomal banding patterns of the hyperdiploid Ehrlich mouse carcinoma (EC) and the Yoshida rat sarcoma (YS) were studied by the quinacrine fluorescence and Giemsa banding methods. The banded karyotypes of both tumors deviated greatly from those of normal somatic cells of host animals. Although most EC cells possessed 43 chromosomes including 1 A, 2 B, and 3 m markers, the banded profile of the karyotype was highly variable. There were usually 26 abnormal elements in which the origin was untraceable even with banding analysis. Of the remaining 17 elements, 14 exhibited apparently normal morphology with normal banding patterns, whereas 3 showed partially normal patterns. The stem line cells of YS had 40 chromosomes, 12–13 apparently normal and 27–28 structurally altered elements. Among the abnormal elements, 9 were traceable either totally or partially. The cell population of YS was more homogeneous compared to that of EC.