Determination of Amino Acids in Serum by Gas Liquid Chromatography

Abstract

Determination of 22 amino acids, including asparagine and glutamine, in serum by GLC of N-isobutyloxycarbonyl (N-isoBOC) methyl ester derivatives was carried out. Serum samples were deproteinized with picric acid and purified with a cation-exchange resin. The amino acids obtained were derived to their N-isoBOC methyl esters and analyzed by GLC. Recoveries of amino acids added to sera ranged 93.1-100.5%; an exception was tyrosine which gave 70.3%. The data obtained by the GLC method were in good agreement with those by the automated amino acid analyzer.