The Control of Transpiration. Insights from Arabidopsis

Abstract

Stomatal complexes in the epidermes of aerial plant parts are critical sites for the regulation of gas ex- change between the plant and the atmosphere. Sto- mata consist of microscopic pores, each flanked by a pair of guard cells. Guard cells can increase or de- crease the size of the pore via changes in their turgor status, hence regulating both CO2 entry into the leaf and transpiration, or the loss of water from the leaf. This Update focuses on recent progress in our under- standing of the regulation of transpiration and drought tolerance that has been garnered through the use of Arabidopsis (Arabidopsis thaliana) as a model experimental system. The coordinated regulation of gas exchange is inte- gral to land plant survival because CO2 must be able to penetrate the leaf to allow photosynthesis, yet water loss (transpiration) must be minimized to prevent des- iccation, drought stress, and plant death. Transpiration also provides the driving force for the transport of water and nutrients from the roots to the aerial tissues, and the evaporation of water from the substomatal cavity cools the plant (Lambers et al., 1998). While a number of morphological traits can contribute to the overall level of leaf gas exchange (e.g. the density and distribution of stomata, leaf epidermal structure and internal organization, cuticle thickness), the regulation of stomatal aperture size is unique in that it is a dynamic and reversible process by which water loss and CO2 influx can be rapidly fine tuned in response to a number of environmental and intrinsic signals, such as light, CO2, and the plant stress hormone abscisic acid (ABA; Schroeder et al., 2001). Because guard cells integrate and respond to a plethora of signals, they have become a model cell type in the field of plant cell signaling (Blatt, 2000; Schroeder et al., 2001; Roelfsema and Hedrich, 2005). This Update highlights recent research reports on the guard cell physiology of Arabidopsis that include some quantitative measure of stomatal function. These measures include transpiration, stomatal conductance (stomatal conductance is defined as stomatal transpi- ration divided by the vapor pressure difference be- tween the leaf and the air, and increases with increasing stomatal aperture), leaf water status, and water-use efficiency/transpiration efficiency (the ratio of photosynthetic assimilation to transpiration). By focusing the article in this manner, we hope to pro- mote the synthesis of ideas and approaches between whole-plant physiologists and molecular biologists/ geneticists. The former typically measure stomatal regulation of gas exchange and its impact on whole- plant physiology, and may treat the cellular and mo- lecular biology of guard cells as a ''black box'' that receives and reacts to inputs. The latter typically use model plant species to investigate cell and molecular regulation of guard cell function, and may employ gene expression, stomatal aperture, or a specific guard cell parameter, such as ion fluxes, as a ''readout,'' without quantifying alterations in gas exchange and concomitant whole-plant impacts. Our premise is that Arabidopsis is an excellent reference plant in which these complementary approaches can be readily com- bined, and that such an integrated approach has great potential to yield new insights into the biology of transpiration in C3 angiosperms.