Nuclear Overhauser studies of CCGGAp, ACCGGp, and ACCGGUp

Abstract

Nuclear Overhauser effect (NOE) measurements are reported for the nonexchangeable base and ribose 1'' proton resonances of CCGGAp, ACCGGp, and ACCGGUP. The results permit assignment of these resonances to particular nucleotides in the sequences. The NOE data rule out conformations containing syn glycosidic linkages and conformations in which the purine 8 and pyrimidine 6 protons are closer to the 3'' nearest-neighbor ribose 1'' proton than to the 5'' nearest-neighbor ribose 1'' proton.