ERYTHROCYTE PRESERVATION. III. THE REVERSIBILITY OF THE STORAGE LESION 1

Abstract

The biochemical storage lesion of the erythrocyte has been shown to be rapidly reversible, once the erythrocyte is introduced into active circulation. After its reconstruction, the erythrocyte also regains its ability to withstand further storage. The 2 major criteria of the storage lesion studied herein were the alterations in erythrocyte phosphate compounds, notably adenosinepolyphosphates, and the ability of the cells to survive in the circulation after transfusion. Certain in vitro manipulations did not produce the reversal demonstrable in vivo, and extra-circulatory in vivo expts. showed only limited regeneration. Implications of this reversibility are discussed.