Resistance of A-431 epidermoid carcinoma cells to early membrane changes induced by tumour promoters
- 1 January 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 3 (7), 837-839
- https://doi.org/10.1093/carcin/3.7.837
Abstract
The A-431 human epidermoid carcinoma cell line was resistant to 12-O-tetradecanoylphorbol-13-acetate (TPA) inhibition of epidermal growth factor binding, enhanced incorporation of [ 3 H]choline into phospholipids and uptake of 86 Rb and [ 3 H]2-deoxyglucose. The cells were also resistant to TPA-stimulated release of radioactive choline derivatives and aracholdonic acid from cells prelabelled with [ 3 H]choline or [ 14 C]arachidonic acid, respectively. The A-431 cells did not metabolise [ 3 H]TPA. Despite their TPA-unresponsiveness, A-431 cells contained specific, high affinity binding sites for [ 3 H]phorbol-12,13-dibutyrate with characteristics similar to other cultured cell lines.This publication has 23 references indexed in Scilit:
- Tetradecanoyl-phorbol acetate stimulates phosphatidylcholine biosynthesis in hela cells by an increase in the rate of the reaction catalyzed by CTP: Phosphocholine cytidylyltransferaseBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1980
- Inhibition of epidermal growth factor binding to cultured mouse epidermal cells by tumor promotersCarcinogenesis: Integrative Cancer Research, 1980
- INHIBITION OF 12-O-TETRADECANOYLPHORBOL-13-ACETATE-ACCELERATED PHOSPHOLIPID-METABOLISM BY 5,8,11,14-EICOSATETRAYNOIC ACID1980
- Specific binding of phorbol ester tumor promotersProceedings of the National Academy of Sciences, 1980
- Phorbol ester induced prostaglandin synthesis and [3H]-TPA metabolism by TPA-sensitive and TPA-resistant friend erythroleukemia cellsBiochemical and Biophysical Research Communications, 1979
- Membrane effects of tumor promoters: Stimulation of sugar uptake in mammalian cell culturesJournal of Cellular Physiology, 1979
- STIMULATION OF CHOLINE INCORPORATION IN CELL-CULTURES BY PHORBOL DERIVATIVES AND ITS CORRELATION WITH THEIR IRRITANT AND TUMOR-PROMOTING ACTIVITY1979
- TUMOR PROMOTER-INDUCED ADHESION OF THE DS19 CLONE OF MURINE ERYTHROLEUKEMIA-CELLS1979
- Tumor-Promoting Phorbol Esters Inhibit Binding of Epidermal Growth Factor to Cellular ReceptorsScience, 1978
- HETEROGENEITY OF MURINE ERYTHROLEUKEMIA CELLS WITH RESPECT TO TUMOR PROMOTER-MEDIATED INHIBITION OF CELL-DIFFERENTIATION1978