TECHNIK UND ERGEBNIS DER HYPOPHYSEKTOMIE BEI DER MAUS

Abstract

After describing in detail the operative technique, the author points out that breed, time of year, and the general health of the animals affect the mortality rate. A few cells of the anterior lobe and of the pars intermedia always remain because during the suction process a central cone of the hypophysis remains attached to the hypophyseal stalk. The histology of both these remnants and the operational field is described. Hypophyseal remnants are present in about 20% of the operated mice even under optimal conditions. Gonadal weight is a rather reliable indication of the hypophyseal remnants even though a dissecting microscope does not disclose them. The absence of large remnants and the probable absence of even small fragments is indicated by seminal vesicular weights of less than 30 mg. Particularly during the 3d and 4th weeks after operation the hypophyseal remnants show an increased secretory function. The thyrotropic action of the remnants is observed only when larger fragments remain. (Larger than those necessary for gonadotropic effect). Post-operative weight loss is prevented only if large remnants are present. The authors conclude that neither the presence of inactive thyroid structure nor decrease in weight are criteria for complete hypophysectomy.