Dose Fractionation and Hepatocyte Clonogens: α/β ≃ 1-2 Gy, and β Decreases with Increasing Delay before Assay

Abstract

The sensitivity to X-ray dose fractionation was assessed for hepatocytes forming colonies in fat pads in mice. When the cells were assayed 1 day after the last irradiation the .alpha./.beta. ratio was 1.0-1.6 depending on the method of analysis. The .alpha./.beta. ratio describing the shape of the single-dose survival curve was much higher, and it did not predict the response to fractionation. When the assay was delayed for 10 months after the fractionated irradiation, the .alpha./.beta. ratio was 1.9-2.1, and the .beta. component showed the greatest change with time. It is concluded that hepatocytes respond to dose fractionation in a manner expected of a late-responding tissue, even when the cells are assayed as early as 1 day after the last dose.