Expression of the mouse dihydrofolate reductase complementary deoxyribonucleic acid in simian virus 40 vectors.
Open Access
- 1 September 1981
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 1 (9), 854-864
- https://doi.org/10.1128/mcb.1.9.854
Abstract
A mouse complementary deoxyribonucleic acid segment coding for the enzyme dihydrofolate reductase has been cloned in two general classes of vectors containing simian virus 40 deoxyribonucleic acid: (i) those that can be propagated as virions in permissive cells and (ii) those that can be introduced into and maintained stably in various mammalian cells. Both types of vectors express the mouse dihydrofolate reductase by using signals supplied by simian virus 40 deoxyribonucleic acid sequences. Moreover, plasmid vectors carrying the complementary deoxyribonucleic acid segment can complement Chinese hamster ovary cells lacking dihydrofolate reductase.This publication has 36 references indexed in Scilit:
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