Reduction of Astrogliosis by Early Treatment of Pneumococcal Meningitis Measured by Simultaneous Imaging, In Vivo, of the Pathogen and Host Response
Open Access
- 1 December 2005
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 73 (12), 7836-7843
- https://doi.org/10.1128/iai.73.12.7836-7843.2005
Abstract
We developed a method for simultaneous in vivo biophotonic monitoring of pneumococcal meningitis and the accompanying neuronal injury in live transgenic mice. Streptococcus pneumoniae engineered for bioluminescence ( lux ) was used for direct visualization of disease progression and antibiotic treatment in a mouse model of meningitis. The host response was monitored in transgenic mice containing an inducible firefly luciferase ( luc ) reporter gene under transcriptional control of the mouse glial fibrillary acidic protein (GFAP) promoter. Based on the different spectra of light emission and substrate requirements for lux and luc , we were able to separately monitor the two reporters using a highly sensitive in vivo imaging system. The level of neuronal damage and recovery following antibiotic treatment was dependent on the time of treatment. This model has potential for simultaneous multiparameter monitoring and testing of therapies that target the pathogen or host response to prevent neuronal injury and recovery.Keywords
This publication has 37 references indexed in Scilit:
- The Dark Side of GliaScience, 2005
- Noninvasive Monitoring of Pneumococcal Meningitis and Evaluation of Treatment Efficacy in an Experimental Mouse ModelMolecular Imaging, 2005
- β-Lactam antibiotics offer neuroprotection by increasing glutamate transporter expressionNature, 2005
- Non-invasive imaging of GFAP expression after neuronal damage in miceNeuroscience Letters, 2004
- The Intermediate Filament GFAP Is Important for the Control of Experimental MurineStaphylococcus aureus-Induced Brain Abscess andToxoplasmaEncephalitisJournal of Neuropathology and Experimental Neurology, 2004
- Glial fibrillary acidic protein gene promoter is differently modulated by transforming growth factor‐beta 1 in astrocytes from distinct brain regionsEuropean Journal of Neuroscience, 2004
- Reprogramming the Host Response in Bacterial Meningitis: How Best To Improve Outcome?Clinical Microbiology Reviews, 2003
- Molecular Imaging of Gene Expression and Efficacy following Adenoviral-Mediated Brain Tumor Gene TherapyMolecular Imaging, 2002
- GFAP Transgenic MiceMethods, 1996
- Spatial memory and adaptive specialization of the hippocampusTrends in Neurosciences, 1992