Notizen: Die Bildung von Plaques durch das Virus der Maul- und Klauenseuche unter methylcellulose-haltigen Medien

Abstract

The use of methyl cellulose (tylose) in place of agar, in addition to a simplification of the technique, provides some other advantages which made it suitable for extending the range of application of the plaque method. The most important among these is that the viscosity of the medium can be adjusted and controlled at will, an important factor for the formation of plaques. By increasing the concentration of tylose the process of plaque formation can be prolonged which makes it possible to study factors which influence it. During the first 48 hours after infection plaque formation by the virus proceeded wihtout disturbance and was not affected apparently by lack of nutrients for the cells or other factors.