Transduction of inositol-fermenting ability demonstrating phylogenetic relationships among strains ofSalmonella typhimurium

Abstract

SUMMARY: The production of Inl⁺recombinants was readily demonstrated in transductional crosses from Inl⁺donor strains to Inl⁻recipient strains from different biotypes and biogroups. None of numerous crosses between different pairs of strains from biotypes 25 and 26 and the biogroup FIRN (biotypes 29, 30, 31 and 32) gave Inl⁺recombinants suggesting that theinlmutation was present at the same intragenic site in all of these strains. Strains of the FIRN biogroup (Fim⁻Inl⁻Rha⁻Bxyl⁻) are thought to have descended by successiverhaandfimmutations from an ancestral bacterium of biotype 25 (Fim⁺Inl⁻Rha⁺Bxyl⁻). The sites of theinlmutations in other Inl⁻biotypes (9, 10, 27 and 25hi) were independent and each was different from that in strains from biotypes 25, 26 and FIRN.