r-DNA plasmid from Schizosaccharomyces pombe: Cloning and use in yeast transformation
- 1 October 1982
- journal article
- Published by Springer Nature in Current Genetics
- Vol. 6 (1), 31-38
- https://doi.org/10.1007/bf00397639
Abstract
An extrachromosomal, extramitochondrial DNA circle of about 3 μm length, occurring in S. pombe, was cloned in pBR322 and a detailed restriction map of the cloned sequence constructed. Hybridization of this DNA with the ribosomal DNA from S. cerevisiae revealed regions of homology which were mapped in the cloned S. pombe DNA. A transformation system was developed using the URA3 gene of S. cerevisiae and the 3 μm molecule. It was shown that the r-DNA part of the hybrid plasmid promotes replication in S. pombe.This publication has 19 references indexed in Scilit:
- Curing of Saccharomyces cerevisiae 2-?m DNA by transformationCurrent Genetics, 1981
- 3 μm DNA — an extrachromosomal ribosomal DNA in the yeast Saccharomyces cerevisiaeGene, 1980
- Nucleotide sequence of the yeast plasmidNature, 1980
- 2 ?m plasmid copy number in different yeast strains and repartition of endogenous and 2 ?m chimeric plasmids in transformed strainsCurrent Genetics, 1980
- Qualitative detection of penicillinase produced by yeast strains carrying chimeric yeast‐coli plasmidsFEBS Letters, 1979
- Insertion of a genetic marker into the ribosomal DNA of yeastPlasmid, 1979
- 2 μm Covalently closed non-mitochondrial circular DNA in the petite-negative yeast Schizosaccharomyces pombeMolecular Genetics and Genomics, 1979
- EcoRI restriction endonuclease cleavage site map of bacteriophage P22 DNAJournal of Molecular Biology, 1978
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975