Modulating effects of opioids, purine compounds, 5-hydroxytryptamine and prostaglandin E2 on cholinergic neurotransmission in a guinea-pig oesophagus preparation

Abstract

The submucous plexus-longitudinal muscularis mucosae preparation of the guinea-pig oesophagus was used to study the actions of morphine, opioid peptides, purine compounds, 5-hydroxytryptamine (5-HT) and prostaglandin E2 (PGE2) on electrically-induced twitch contractions which are probably mediated by cholinergic nerve stimulation. The twitch contractions were inhibited by morphine (1–100 μM), methionine-enkephalin (1–100 μM) and β-endorphin (0.1–1 μM), but increased by adenosine (1–30 μM), adenosine 5′-triphosphate (1–30 μM), 5-HT (0.01–3 μM) and PGE2 (1–10 nM). The submaximal contraction induced by acetylcholine (12 or 20 nM) which is nearly equivalent to the twitch contractions was unaffected by morphine, methionine-enkephalin, β-endorphin and 5-HT, but augmented by purine compounds and PGE2. It is concluded that cholinergic neurotransmission in the submucous plexus-longitudinal muscularis mucosae of the guinea-pig oesophagus is inhibited by morphine and opioid peptides acting at prejunctional opiate receptors, and facilitated by 5-HT, purine compounds and PGE2 via prejunctional or postjunctional mechanisms.