Unit gravity sedimentation separation of cells comprising the caput epididymidis of the rat
- 1 September 1977
- journal article
- research article
- Published by Springer Nature in Cell and tissue research
- Vol. 183 (3), 371-378
- https://doi.org/10.1007/bf00220643
Abstract
Summary Cells from the rat caput epididymidis were separated by unit gravity sedimentation. Purest 12-ml fractions contained 88–95% basal cells or 64–76% principal cells. Ultrastructure of separated cells was similar to that of cells in intact tissue. Viability of separated cells was excellent as determined by dye exclusion tests and cellular ATP content. By combining fractions pools containing 4.0±0.9 × 106 cells (86±8% basal cells) and 1.4±0.4 × 106 cells (56±7% principal cells) were obtained. Thus, studies on the function of basal and principal cells from the rat caput epididymidis should be possible.Keywords
This publication has 3 references indexed in Scilit:
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- INTRAEPITHELIAL LYMPHOCYTES IN THE MALE REPRODUCTIVE TRACT OF RATS AND RHESUS MONKEYSReproduction, 1975
- The ultrastructure of the principal cells and intraepithelial leucocytes in the initial segment of the rat epididymisThe Anatomical Record, 1973