Diamidino yellow dihydrochloride (DY·2HCl); a new fluorescent retrograde neuronal tracer, which migrates only very slowly out of the cell
- 1 July 1983
- journal article
- research article
- Published by Springer Nature in Experimental Brain Research
- Vol. 51 (2), 179-191
- https://doi.org/10.1007/bf00237193
Abstract
Earlier studies showed that Nuclear Yellow (NY), True Blue (TB) and Fast Blue (FB) are transported retrogradely through axons to their parent cell bodies. NY produces a yellow fluorescent labeling of the neuronal nucleus at 360 nm excitation wavelength, while TB and FB produce a blue fluorescence of the cytoplasm at this same wavelength. Therefore, NY may be combined with TB or FB in double-labeling experiments demonstrating the existence of axon collaterals. However, retrograde neuronal labeling with TB or FB requires a relatively long survival time, while NY requires a short survival time since NY migrates rapidly out of the retrogradely labeled neurons. This complicates double-labeling experiments since TB and FB must be injected first and NY later, a short time before the animal is sacrificed. We report a new yellow fluorescent tracer which labels mainly the nucleus and migrates much more slowly out of the retrogradely labeled neurons than NY. This new tracer can be used instead of NY in combination with TB or FB in double-labeling experiments and unlike NY can be injected at the same time as TB or FB. The new tracer is a diamidino compound (no. 28826) which is commercially Distributed by Dr. Illing KG, Warthweg 14-18, Postfach 1150, D-6114 Groß-Umstadt, FRG available. It will be referred to as Diamidino Yellow Dihydrochloride (DY·2HCl). According to the present study DY·2HCl is transported over long distances in rat and cat, and produces a yellow fluorescence of the neuronal nucleus at 360 nm excitation wavelength, resembling that obtained with NY. When combined with TB or FB, DY·2HCl is as effective as NY in double labeling of neurons by way of divergent axon collaterals. Distributed by Dr. Illing KG, Warthweg 14-18, Postfach 1150, D-6114 Groß-Umstadt, FRGThis publication has 24 references indexed in Scilit:
- Collaterals of rubrospinal neurons to the cerebellum in rat. A retrograde fluorescent double labeling studyBrain Research, 1983
- Electrophysiological evidence for branching nigral projections to pontine reticular formation, superior colliculus and thalamusBrain Research, 1982
- A search for corticospinal collaterals to thalamus and mesencephalon by means of multiple retrograde fluorescent tracers in cat and ratBrain Research, 1981
- Quantitative differences in collateralization of the descending spinal pathways from red nucleus and other brain stem cell groups in rat as demonstrated with the multiple fluorescent retrograde tracer techniqueBrain Research, 1981
- Evidence for a major spinal cord projection from the diencephalic A11 dopamine cell group in the rat using transmitter-specific fluorescent retrograde tracingBrain Research, 1979
- Simultaneous use of retrograde fluorescent tracers and fluorescence histochemistry for convenient and precise mapping of monoaminergic projections and collateral arrangements in the CNSJournal of Neuroscience Methods, 1979
- The organization of the efferent projections of the substantia nigra in the rat. A retrograde fluorescent double labeling studyBrain Research, 1979
- The organization and postnatal development of the commissural projection of the rat somatic sensory cortexJournal of Comparative Neurology, 1976
- The cells of origin of the corpus callosum in rat, cat and rhesus monkeyBrain Research, 1974
- Acetylcholinesterase activity in the red nucleus of the rat. Effects of rubrospinal tractotomyBrain Research, 1971