Ethyl Acetate Fraction of Adlay Bran Ethanolic Extract Inhibits Oncogene Expression and Suppresses DMH-Induced Preneoplastic Lesions of the Colon in F344 Rats through an Anti-inflammatory Pathway
- 10 June 2010
- journal article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 58 (13), 7616-7623
- https://doi.org/10.1021/jf101084e
Abstract
Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) is a grass crop and was reported to possess anti-inflammatory activity and an antiproliferative effect in cancer cell lines. The purpose of this study was to evaluate the effects of the ethyl acetate fraction of an adlay bran ethanolic extract (ABE-Ea) on colon carcinogenesis in an animal model and investigate its mechanism. Male F344 rats received 1,2-dimethylhydrazine (DMH) and consumed different doses of ABE-Ea. The medium-dose group (17.28 mg of ABE-Ea/day) exhibited the best suppressive effect on colon carcinogenesis and prevented preneoplastic mucin-depleted foci (MDF) formation. Moreover, RAS and Ets2 oncogenes were significantly down-regulated in this group compared to the negative control group, whereas Wee1, a gene involved in the cell cycle, was up-regulated. Cyclooxygenase-2 (COX-2) protein expression was significantly suppressed in all colons receiving the ABE-Ea, indicating that ABE-Ea delayed carcinogenesis by suppressing chronic inflammation. ABE-Ea included considerable a proportion of phenolic compounds, and ferulic acid was the major phenolic acid (5206 μg/g ABE-Ea) on the basis of HPLC analysis. Results from this study suggest that ABE-Ea suppressed DMH-indued preneoplastic lesions of the colon in F344 rats and that ferulic acid may be one of the active compounds.Keywords
This publication has 25 references indexed in Scilit:
- Effect of Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) Testa and Its Phenolic Components on Cu2+-Treated Low-Density Lipoprotein (LDL) Oxidation and Lipopolysaccharide (LPS)-Induced Inflammation in RAW 264.7 MacrophagesJournal of Agricultural and Food Chemistry, 2009
- Frequent Mutation of Apc Gene in Rat Colon Tumors and Mucin-Depleted Foci, Preneoplastic Lesions in Experimental Colon CarcinogenesisCancer Research, 2007
- Increased protein expression of both inducible nitric oxide synthase and cyclooxygenase-2 in human colon cancersCancer Letters, 2006
- Effects of adlay on azoxymethane-induced colon carcinogenesis in ratsFood and Chemical Toxicology, 2004
- Most Effective Colon Cancer Chemopreventive Agents in Rats: A Systematic Review of Aberrant Crypt Foci and Tumor Data, Ranked by PotencyNutrition and Cancer, 2002
- Sialomucin production in aberrant crypt foci relates to degree of dysplasia and rate of cell proliferationCancer Letters, 2001
- Lifestyle and Colon Cancer: An Assessment of Factors Associated with RiskAmerican Journal of Epidemiology, 1999
- Cyclooxygenase 2 is induced in colonic epithelial cells in inflammatory bowel diseaseGastroenterology, 1998
- Components of the AIN-93 Diets as Improvements in the AIN-76A DietJournal of Nutrition, 1997
- Risk factors for colon neoplasia—epidemiology and biologyEuropean Journal Of Cancer, 1995