Recombinant platelet-derived growth factor B gene expression in porcine arteries induce intimal hyperplasia in vivo.
Open Access
- 1 April 1993
- journal article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 91 (4), 1822-1829
- https://doi.org/10.1172/jci116394
Abstract
Platelet-derived growth factor (PDGF) B chain induces cell proliferation in vitro and is associated with arterial lesions that cause cardiovascular disease. However, it has been difficult to document the biological response to PDGF B gene expression in arteries in vivo. To determine the biologic effects of this growth factor in vivo, we have introduced an eukaryotic expression vector plasmid encoding recombinant PDGF B by direct gene transfer into porcine iliofemoral arteries using DNA liposome complexes. The presence of PDGF B plasmid DNA and expression of recombinant mRNA were confirmed by polymerase chain reaction analysis, and recombinant PDGF protein was demonstrated by immunohistochemistry. Intimal thickening was observed in porcine arteries 21 days following transfection with the recombinant PDGF B gene compared with arteries transduced with a control gene, E. coli beta-galactosidase. An eightfold increase in intimal to medial ratio was present in PDGF B gene transfected arteries compared with control transfected arteries (P = 0.001). This study suggests that expression of a recombinant PDGF B gene in vivo can play a role in the induction of intimal hyperplasia, which can lead to cardiovascular diseases.Keywords
This publication has 18 references indexed in Scilit:
- Percutaneous arterial gene transfer in a rabbit model. Efficiency in normal and balloon-dilated atherosclerotic arteries.JCI Insight, 1992
- Cell proliferation in human coronary arteries.Proceedings of the National Academy of Sciences, 1990
- Recombinant Gene Expression in Vivo Within Endothelial Cells of the Arterial WallScience, 1989
- Platelet-derived growth factor mRNA detection in human atherosclerotic plaques by in situ hybridization.JCI Insight, 1988
- Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer.Proceedings of the National Academy of Sciences, 1987
- The biology of platelet-derived growth factorCell, 1986
- KINETICS OF CELLULAR PROLIFERATION AFTER ARTERIAL INJURY .3. ENDOTHELIAL AND SMOOTH-MUSCLE GROWTH IN CHRONICALLY DENUDED VESSELS1986
- Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assaysJournal of Immunological Methods, 1983
- Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequencesNature, 1981
- Calcium as a Regulator of the Proliferation of Normal, but Not of Transformed, Chicken Fibroblasts in a Plasma-Containing MediumProceedings of the National Academy of Sciences, 1971