Establishment and Cytogenetic Analysis of a Cell Line Derived from a Human Epithelioma of the Lung

Abstract

A continuously growing cell line, established from a bronchial carcinoma of squamous cell type derived from a 56 yr old white male, has been kept in culture since 1971. The line, named E14, is now in its 158th passage. Cytogenetic analysis demonstrates a slight gain of chromosome number up to the 100th passage. Since then the stem line has remained fairly stable at between 58 and 61 chromosomes. Banding analyses indicate that, whereas minor changes in the structural rearrangements of the karyotype are constantly occurring, the basic tumor genotype remains fairly stable.