Studies on the Exchange of Fluids Between Host and Tumor. I. A Method for Growing “Tissue-Isolated” Tumors in Laboratory Animals2

Abstract

A technique is described which provides the investigator with a growing tumor enveloped in paraffin and thus isolated from the surrounding tissues but connected with the host by a single artery and vein from which blood can be withdrawn. The procedure can be applied to all tumors normally grown in a solid form in rats, hamsters, and mice. Two transplants of the same tumor, one grown isolated in the paraffin envelope and the other grown subcutaneously, showed the same correlation between wet weight, dry weight, and total nitrogen, as well as the same level of anaerobic glycolysis. Animals bearing the “isolated” transplants behaved in the same manner as those bearing subcutaneous tumors in regard to body weight, food consumption, and water intake; liver catalase was also equally depressed.