NADP‐Dependent Isocitrate Dehydrogenase from the Mussel Mytilus edulis L.
- 1 October 1980
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 111 (2), 575-579
- https://doi.org/10.1111/j.1432-1033.1980.tb04973.x
Abstract
A purification scheme is described for NADP-dependent isocitrate dehydrogenase from the digestive gland of M. edulis. The scheme incorporates 3 chromatographic steps: hydroxyapatite adsorption, blue-Sepharose affinity chromatography and DEAE-Sephacel ion-exchange chromatography. The subunit MW of the enzyme was 45000 (.+-. 5000) by sodium dodecylsulfate gel electrophoresis. The purified enzyme was homogeneous on dodecylsulfate gels and had a specific activity of 30-50 U/mg protein.This publication has 20 references indexed in Scilit:
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