Determination of oxalate in human serum by multicolumn isotachophoresis

Abstract

The practical usefulness of multicolumn isotachophoresis was demonstrated by the determination of oxalate levels in human serum. 10 mmol/L HC1 and 100 mmol/L Na₂HPO₄ served as the leading and terminating electrolyte, respectively. For the stabilization of the isotachophoretic zones in large cross section channels a suspension of Bio‐Gel P‐300 in the leading electrolyte was used. For the analysis ca. 1 mL of fresh serum was required and 1.2 mL of 1:1 mixture of serum and the suspension of Bio‐Gel P‐300 in deionized water was applied as sample. The time of analysis ranged between 45–49 min. The detection limit of analysis was determined to be 50 nmol/L. Reproducibility of analysis of 1 μmol/L oxalate in water standard was found to be 2.6% (n = 6).