Direct evidence for a xylose metabolic pathway in Saccharomyces cerevisiae

Abstract

Xylose transport, xylose reductase, and xylitol dehydrogenase activities are demonstrated in Saccharomyces cerevisiae. The enzymes in the xylose catabolic pathway necessary for the conversion of xylose to xylulose are present, although S. cerevisiae cannot grow on xylose as a sole carbon source. Xylose transport is less efficient than glucose transport, and its rate is dependent upon aeration. Xylose reductase appears to be a xylose inducible enzyme and xylitol dehydrogenase activity is constitutive, although both are repressed by glucose. Both xylose reductase and xylitol dehydrogenase activities are five– to tenfold lower in S. cerevisiae as compared to Candida utilis. In vivo conversion of ¹⁴C-xylose in S. cerevisiae is demonstrated and xylitol is detected, although no significant levels of any other ¹⁴C-labeled metabolites (e. g., ethanol) are observed.