Elution of Pollen Antigens from Tannic Acid Treated Erythrocytes.
- 1 October 1957
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 96 (1), 71-73
- https://doi.org/10.3181/00379727-96-23396
Abstract
Using the tannic acid hemagglutination technique of Boyden it was observed that short ragweed antigens slowly eluted from the red cells and in so doing, caused a decrease in hemagglutination titer with time. Upon removal of the eluted antigen in the supernatant by washing, the cells again were capable of agglutinating to full titer for short periods. A crude fractionation of the antigen with trichloracetic acid precipitation and with dialysis yielded some antigenic fractions which gave very little elution while others were strongly elutable. The presence of antiserum was not required for this elution to occur.Keywords
This publication has 3 references indexed in Scilit:
- The Detection of Antibodies in Hayfever Sera by Means of HemagglutinationThe Journal of Immunology, 1956
- THE ADSORPTION OF PROTEINS ON ERYTHROCYTES TREATED WITH TANNIC ACID AND SUBSEQUENT HEMAGGLUTINATION BY ANTIPROTEIN SERAThe Journal of Experimental Medicine, 1951
- Adsorption of Bacterial Polysaccharides to ErythrocytesNature, 1948