Quantitative IR spectrophotometry of peptide compounds in water (H2O) solutions. III. Estimation of the protein secondary structure
- 1 January 1990
- journal article
- research article
- Published by Wiley in Biopolymers
- Vol. 30 (13-14), 1273-1280
- https://doi.org/10.1002/bip.360301311
Abstract
Infrared spectra of 13 globular proteins have been obtained in the 1800–1480-cm−1 region for H2O solutions. A method for estimating protein secondary structure from the ir spectrum has been developed. The method can also be used for estimating polypeptide and fibrous protein conformation. For the globular and fibrous proteins and polypeptides analyzed, the correlation coefficients between the ir and x-ray estimates of ordered helix, disordered helix, ordered β-structure, disordered β-structure, turns, and remainder were 0.98, 0.80, 0.99, 0.87, 0.90, and 0.92 respectively.Keywords
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