The incidence of spontaneous cytogenetic aberrations in lymphocytes cultured from normal humans for 48 and 72 h
- 1 October 1984
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Genetics and Cytology
- Vol. 26 (5), 528-531
- https://doi.org/10.1139/g84-084
Abstract
Spontaneously occurring cytogenetic aberrations were compared in 48- and 72-h human lymphocyte cultures from a group of 20 normal male volunteers. Mitotic indices for both cultures times were also evaluated. BUdR (5-bromo, 2-deoxyuridine) labelled metaphases from three men were utilized to determine the cell cycle kinetics in the medium used. There was no statistically significant difference between 48- and 72-h cultures among the majority of aberration categories considered. Possibly, the lymphocyte populations at the two different culture times maintained a consistent yield level. Mitotic indices in 72-h cultures were invariably higher than those in 48-h cultures. An overwhelming number of first division metaphases were observed at 48 h of incubation; however, approximately 22% of the lymphocytes did not enter into mitosis until 72 h in culture. A number of difficulties with regard to utilizing BUdR-labelled first division metaphases for cytogenetic analyses are discussed.Key words: BUdR, lymphocytes, mitosis, culture.This publication has 13 references indexed in Scilit:
- Chromosome changes in lymphocytes after occupational exposure to pentachlorophenol (PCP)Mutation Research/Genetic Toxicology, 1982
- Frequency and types of induced and spontaneous chromosome aberrations in relation to cell kineticsHuman Genetics, 1981
- Cell cycle kinetics in human lymphocyte culturesNature, 1980
- Time of first-generation metaphasesMutation Research, 1980
- Frequencies of sister-chromatid exchanges in relation to cell kinetics in lymphocyte culturesMutation Research, 1980
- The influence of culture medium composition on the incidence of chromosomal breakageHuman Genetics, 1979
- The utilization of bromodeoxyuridine incorporation into DNA for the analysis of cellular kineticsExperimental Cell Research, 1976
- Cell cycle analysis in asynchronous cultures using the BUdR-Hoechst techniqueExperimental Cell Research, 1976
- The human leukocyte test systemHuman Genetics, 1976
- Chromosome preparations of leukocytes cultured from human peripheral bloodExperimental Cell Research, 1960