Three-dimensional structural model of eubacterial 5S RNA that has functional implications.
- 1 August 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (15), 4599-4603
- https://doi.org/10.1073/pnas.79.15.4599
Abstract
Escherichia coli 5S RNA and its specific protein complexes were hydrolyzed with the single-strand-specific nuclease S1. A tertiary structural model for E. coli 5S RNA is proposed in which ribosomal proteins E-L5, E-L18 and E-L25 influence the conformation of the RNA. This may be of significance for ribosomal function. Comparison of the proposed E. coli 5S RNA structure with those of 18 other prokaryotic 5S RNA led to a generalized eubacterial 5S RNA tertiary structure in which the majority of the conserved nucleotides are in non-base-paired regions and several conserved looped-out adenines (in E. coli, adenines -52, -53, -57, -58 and -66) could be important for protein recognition or interaction or both.This publication has 35 references indexed in Scilit:
- A slow tritium exchange study of the solution structure of Escherichia coli 5 S ribosomal RNAJournal of Molecular Biology, 1981
- Laser Raman studies of rat liver ribosomal 5 S RNAFEBS Letters, 1981
- A general model for the conformational switch in 5S RNA during protein synthesisJournal of Theoretical Biology, 1980
- Structures of complexes of 5S RNA with ribosomal proteins L5, L18 and L25 from Escherichia coli: Identification of kethoxal-reactive sites on the 5S RNAJournal of Molecular Biology, 1979
- Structure and macromolecular interactions of 5S RNA. 1. Stoichiometry, cooperativity, and stability of interactions between 5S RNA and proteins L5, L18, and L25 from the 50S ribosomal subunit of Escherichia coliBiochemistry, 1978
- Binding oligonucleotides to Escherichia coli and Bacillus stearothermophilus 5 S RNAJournal of Molecular Biology, 1978
- Identification of the single-strand regions in Escherichia coli 5S RNA, native and A forms, by the binding of oligonucleotidesBiochemistry, 1977
- Molecular Model for 5‐S RNAEuropean Journal of Biochemistry, 1976
- A fragment of 23S RNA containing a nucleotide sequence complementary to a region of 5S RNAFEBS Letters, 1975
- Activities of B. stearothermophilus 50 S ribosomes reconstituted with prokaryotic and eukaryotic 5 S RNAFEBS Letters, 1973