Dissociation of the ligand and dephosphorylation of the platelet‐derived growth factor receptor
- 20 June 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 233 (2), 371-374
- https://doi.org/10.1016/0014-5793(88)80463-0
Abstract
The ligand-induced phosphorylation of the platelet-derived growth factor (PDGF) receptor was followed at 37°C by a rapid dephosphorylation which was roughly parallel to the down regulation of the 125I-PDGF binding sites. At 4°C, when the ligand-receptor complexes remain associated with the cell surface, the phosphorylated form of the receptor was more stable. However if the ligand was dissociated from the receptor by means of a mild acid wash or a treatment with suramin, the dephosphorylation of the receptor also occurred at a low temperature. These data suggest that, due to the dissociation of the ligand, the kinase activity of the receptor is switched off so that the phosphotyrosine-containing receptors remain exposed to the action of phosphatases that rapidly dephosphorylate them.Keywords
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