ESTABLISHMENT OF A CELL LINE (CO-115) FROM A HUMAN COLON CARCINOMA TRANSPLANTED INTO NUDE MICE

Abstract

A human colon carcinoma cell line, Co-115, was established in vitro from solid xenografts maintained in nude mice and subcultured for 95 passages. Co-115 cells grown in vitro as tightly packed, epithelial-like colonies, had a doubling time of about 36 h, a relatively low plating efficiency in agar and released significant amounts of carcinoembryonic antigen to the culture medium. Their epithelial nature was confirmed by ultrastructural examination. The injection of Co-115 cells into nude mice reinduced the formation of solid tumor masses that could be retransplanted and showed a morphology comparable to that of the original xenograft.