Constitutive Expression of a 2′,5′-Oligoadenylate Synthetase cDNA Results in Increased Antiviral Activity and Growth Suppression
- 1 December 1989
- journal article
- research article
- Published by Mary Ann Liebert Inc in Journal of Interferon Research
- Vol. 9 (6), 649-657
- https://doi.org/10.1089/jir.1989.9.649
Abstract
The interferon (IFN)-induced enzyme 2′,5′-oligoadeny late (2-5A) synthetase has been implicated in the development of antiviral activity in human and animal cells. However, its role in IFN-mediated growth inhibition remains unclear. To elucidate the function of 2-5A synthetase, we have stably introduced a human 2-5A synthetase cDNA into a human glioblastoma cell line (T98G). Constitutive expression of the cDNA in these cells is associated with increased levels of resistance to infection by encephalomyocarditis virus. One transfected subclone, which expresses elevated levels of 2-5A synthetase enzyme activity, also shows a reduced rate of cellular proliferation.This publication has 36 references indexed in Scilit:
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